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The 80-wire (but still 40-pin connector!) cable supports ATA-66 and faster. I recall reading that the increased number of wires reduce "signal noise" allowing for shorter data "chunks" being sent, so more "chucnks" of data can be sent in the same period of time (my paraphrasing may be way off).
The 80-wire cable won't do anything for an ATA-33 device. The 80-wire cable also won't do anything if your motherboard only supports ATA-33 devices and is not manufactured to the ATA-66 (or faster) standard.
If you have ATA-66 hardware and motherboard, and have been running ATA-33 (40-wire) cable, then the new cable should provide a performance increase.
You may need to check settings in Windows Device Manager to make sure your devices are running at the correct speeds.
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